Enzyme digestion based 4C-Seq

w4CSeq applies a customized pipeline to deal with enzyme digestion based 4C-Seq data, identifies statistically significant regions, draws interacting plots for intra-chromosome and inter-chromosome interactions, and overlays genomic features (TSS, TTS, CpG sites), DNA replication timing and user-provided annotation onto it. The combined plot will help uncover significant features in/around 4C regions.

Basic Information

Email (optional)

Input FASTQ file

Genome build

hg18 hg19 mm9 mm10

Specify a bait region

DNA sequence of 4C target region

Bait chromosome

Start position of inverse primer

End position of inverse primer

Parameters for statistical analysis (Note: use count of enzyme sites to represent genomic span)

Bin size for TRANS chromosome

Bin size for CIS chromosome

Window size for CIS chromosome

False Discovery Rate (FDR)

Optional: You can upload bed files for association analysis

Click to upload

Click to add input

file #0 (input)

file #1 Name

file #2 Name

file #3 Name

file #4 Name

file #5 Name

file #6 Name

file #7 Name

file #8 Name

file #9 Name

file #10 Name

Enzyme digestion based 4C-Seq

Basic Information

Specify a bait region

Parameters for statistical analysis (Note: use count of enzyme sites to represent genomic span)

Optional: You can upload bed files for association analysis

Please send questions or comments here