w4CSeq allows for unbiased identification of genome-wide interaction partners with pre-specified "bait" elements or "viewpoints".
w4CSeq is a cost-effective methodology and computational analysis tool for robust characterization of the physical organization around selected functional element using chromosome conformation capture combined with high-throughput sequencing (4C-Seq) technique.
Find orderness from unorderness. Genomic interactions contribute greately to the well-regulated gene expression. See how to use w4CSeq to identify those interactions.
w4CSeq applies a customized pipeline to deal with both enzyme digestion and sonication fragmentation based 4C-seq data, identifies statistically significant regions, draws interacting plots for intra-chromosome and inter-chromosome interactions, and overlays genomic features (TSS, TTS, CpG sites), DNA replication timing and user-provided annotation onto it. The combined plot will help uncover significant features in/around 4C regions. (Note: This website is a demo server for you to examine, thus may not handle large amounts of data.)
Sonication is used to randomly fragment chromatin fibers instead of restriction enzymes at specific sites.
Wanna know more information on 4C-seq and w4CSeq? See the FAQ page to explore more useful points.
