Explore chromosome landscape within nucleus
w4CSeq allows for unbiased identification of genome-wide interaction partners with pre-specified "bait" elements or "viewpoints".
Effectively capture significant chromosomal interactions
w4CSeq is a cost-effective methodology and computational analysis tool for robust characterization of the physical organization around selected functional element using chromosome conformation capture combined with high-throughput sequencing (4C-Seq) technique.
Wander in the zigzag of genomic "forests"
Find orderness from unorderness. Genomic interactions contribute greately to the well-regulated gene expression. See how to use w4CSeq to identify those interactions.
A demo web server to process 4C-Seq data
w4CSeq applies a customized pipeline to deal with both enzyme digestion and sonication fragmentation based 4C-seq data, identifies statistically significant regions, draws interacting plots
for intra-chromosome and inter-chromosome interactions, and overlays genomic features (TSS, TTS, CpG sites), DNA replication timing and user-provided annotation onto it. The
combined plot will help uncover significant features in/around 4C regions.
(Note: This website serves as a demo only. You can establish your own web server and run the command line tool by downloading source codes at https://github.com/WGLab/w4CSeq)
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Enzyme based 4C
Restriction enzyme digestion is used to fragment chromosomes.
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Sonication based 4C
Sonication is used to randomly fragment chromatin fibers instead of restriction enzymes at specific sites.
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Wanna know more information on 4C-seq and w4CSeq? See the FAQ page to explore more useful points.
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Image courtesy of [Linda Tanner, Nicola Perantoni, and James Forbes]